Regulatory

Part:BBa_K1713000:Design

Designed by: Xudong Luo   Group: iGEM15_HZAU-China   (2015-09-14)

Plac/ara-1, a hybrid promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Compared with BBa_K094120, We have 1 site mutation and 1 gap which lead to two more restriction enzyme cutting sites, HindIII and BamHI.

Source

It was reconstructed through PCR with BBa_K094120 served as template and a pair of primers with two mutation.

References

Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements[J]. Nucleic acids research, 1997, 25(6): 1203-1210.